Analysis of the chemical components of hydatid fluid from Echinococcus granulosus
Rev. Soc. Bras. Med. Trop
; 46(5): 605-610, Sept-Oct/2013. tab, graf
Article
de En
| LILACS
| ID: lil-691417
Bibliothèque responsable:
BR1.1
ABSTRACT
Introduction The aim of this study was to explore the environment of Echinococcus granulosus (E. granulosus) protoscolices and their relationship with their host. Methods Proteins from the hydatid-cyst fluid (HCF) from E. granulosus were identified by proteomics. An inductively coupled plasma atomic emission spectrometer (ICP-AES) was used to determine the elements, an automatic biochemical analyzer was used to detect the types and levels of biochemical indices, and an automatic amino acid analyzer was used to detect the types and levels of amino acids in the E. granulosus HCF. Results I) Approximately 30 protein spots and 21 peptide mass fingerprints (PMF) were acquired in the two-dimensional gel electrophoresis (2-DE) pattern of hydatid fluid; II) We detected 10 chemical elements in the cyst fluid, including sodium, potassium, calcium, magnesium, copper, and zinc; III) We measured 19 biochemical metabolites in the cyst fluid, and the amount of most of these metabolites was lower than that in normal human serum; IV) We detected 17 free amino acids and measured some of these, including alanine, glycine, and valine. Conclusions We identified and measured many chemical components of the cyst fluid, providing a theoretical basis for developing new drugs to prevent and treat hydatid disease by inhibiting or blocking nutrition, metabolism, and other functions of the pathogen. .
Mots clés
Texte intégral:
1
Collection:
01-internacional
Base de données:
LILACS
Sujet principal:
Liquide kystique
/
Echinococcus granulosus
/
Échinococcose
Limites:
Animals
/
Humans
Langue:
En
Journal:
Rev. Soc. Bras. Med. Trop
Sujet du journal:
MEDICINA TROPICAL
Année:
2013
Type de document:
Article
Pays d'affiliation:
Chine