Integrin signalling defects in T-lymphocytes in systemic lupus erythematosus.
Lupus
; 8(1): 39-51, 1999.
Article
de En
| MEDLINE
| ID: mdl-10025598
ABSTRACT
OBJECTIVE:
To establish the relationship between T cell responses to integrin coreceptor stimulation and B cell hyperreactivity as measured by pathologic autoantibody production.METHODS:
Peripheral blood mononuclear cells from 42 patients with SLE according to the American Rheumatism Association criteria were examined for their ability to adhere to plate-immobilised fibronectin. Co-stimulation assays were performed on the same cells using anti-CD3 antibody alone or co-immobilised with an anti-beta1-integrin antibody. Proliferative responses were measured by 3[H]thymidine pulsing on day 3 and activation was determined using a commercial protein kinase C assay, the protocol being established by our group in association with Promega. Beta-integrin expression was established by FACS analysis.RESULTS:
An impaired PKC response to integrin-mediated activation was found in T-lymphocytes from 6/21 (29%) SLE patients, which correlated significantly with an absence of anti-dsDNA antibody in patient sera, irrespective of prednisolone treatment. Integrin co-stimulation of TcR/CD3-induced proliferation and T cell adhesion to fibronectin were also impaired among 5/21 (24%) and 6/15 (40%) patients studied, respectively.CONCLUSION:
We hypothesise that the integrity of beta1-integrin signalling pathways may influence pathological antibody production in SLE by affecting T-lymphocyte activation and interactions between T- and B-lymphocytes.
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Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Lymphocytes T
/
Antigènes CD29
/
Lupus érythémateux disséminé
Type d'étude:
Guideline
Limites:
Adolescent
/
Adult
/
Aged
/
Female
/
Humans
/
Male
/
Middle aged
Langue:
En
Journal:
Lupus
Sujet du journal:
REUMATOLOGIA
Année:
1999
Type de document:
Article
Pays d'affiliation:
Royaume-Uni