Differential addressing of 5-HT1A and 5-HT1B receptors in epithelial cells and neurons.
J Cell Sci
; 112 ( Pt 6): 967-76, 1999 Mar.
Article
de En
| MEDLINE
| ID: mdl-10036246
ABSTRACT
The 5-HT1A and 5-HT1B serotonin receptors are expressed in a variety of neurons in the central nervous system. While the 5-HT1A receptor is found on somas and dendrites, the 5-HT1B receptor has been suggested to be localized predominantly on axon terminals. To study the intracellular addressing of these receptors, we have used in vitro systems including Madin-Darby canine kidney (MDCK II) epithelial cells and primary neuronal cultures. Furthermore, we have extended these studies to examine addressing in vivo in transgenic mice. In epithelial cells, 5-HT1A receptors are found on both apical and basolateral membranes while 5-HT1B receptors are found exclusively in intracellular vesicles. In hippocampal neuronal cultures, 5-HT1A receptors are expressed on somatodendritic membranes but are absent from axons. In contrast, 5-HT1B receptors are found on both dendritic and axonal membranes, including growth cones where they accumulate. Using 5-HT1A and 5-HT1B knockout mice and the binary tTA/tetO system, we generated mice expressing these receptors in striatal neurons. These in vivo experiments demonstrate that, in striatal medium spiny neurons, the 5-HT1A receptor is restricted to the somatodendritic level, while 5-HT1B receptors are shipped exclusively toward axon terminals. Therefore, in all systems we have examined, there is a differential sorting of the 5-HT1A and 5-HT1B receptors. Furthermore, we conclude that our in vivo transgenic system is the only model that reconstitutes proper sorting of these receptors.
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Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Encéphale
/
Récepteurs sérotoninergiques
/
Cellules épithéliales
/
Neurones
Limites:
Animals
Langue:
En
Journal:
J Cell Sci
Année:
1999
Type de document:
Article
Pays d'affiliation:
États-Unis d'Amérique