Cell-mediated immune responses to antigens of Bordetella pertussis and protection against pertussis in school children.
Pediatr Infect Dis J
; 18(4): 366-70, 1999 Apr.
Article
de En
| MEDLINE
| ID: mdl-10223692
ABSTRACT
BACKGROUND:
Increasing evidence suggests that cell-mediated immunity (CMI) is involved in immune response against Bordetella pertussis. However, there are practically no studies evaluating the significance of pertussis-specific CMI in relation to protection against clinical pertussis.METHODS:
An outbreak of pertussis was studied prospectively in 13-year-old pupils in a rural school. B. pertussis infection was diagnosed by culture, microagglutination and enzyme immunoassay serology with the use of pertussis toxin, filamentous hemagglutinin and pertactin as antigens. Pertussis-specific CMI responses were assessed by in vitro proliferation assay of peripheral blood mononuclear cells.RESULTS:
At the initial sampling 7 of 22 children had symptoms suggestive of pertussis and 15 were asymptomatic. Of the latter 3 remained healthy, 8 were later confirmed to have had asymptomatic infection, 3 developed laboratory-confirmed pertussis and 1 developed cough without laboratory evidence of pertussis. Initial in vitro proliferations of peripheral blood mononuclear cells induced by pertussis toxin, filamentous hemagglutinin and/or pertactin were positive in all 3 healthy children, in 6 of 8 children who had asymptomatic infection, but in none of the 3 children who later developed pertussis. Although some children who remained healthy had high values of antibodies, no clear association was found between initial serum antibody values and clinical outcome.CONCLUSIONS:
These preliminary data suggest that CMI may have an important role in protection against clinical pertussis but do not exclude a role for antibodies. Furthermore the results stress a multifactorial nature of the immune protection against B. pertussis.
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Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Bordetella pertussis
/
Coqueluche
/
Anticorps antibactériens
/
Antigènes bactériens
Type d'étude:
Diagnostic_studies
/
Observational_studies
/
Risk_factors_studies
Limites:
Adolescent
/
Female
/
Humans
/
Male
Langue:
En
Journal:
Pediatr Infect Dis J
Sujet du journal:
DOENCAS TRANSMISSIVEIS
/
PEDIATRIA
Année:
1999
Type de document:
Article
Pays d'affiliation:
Finlande