Evaluation of a vanA-specific PCR assay for detection of vancomycin-resistant Enterococcus faecium during a hospital outbreak.
J Clin Microbiol
; 37(10): 3348-9, 1999 Oct.
Article
de En
| MEDLINE
| ID: mdl-10488203
ABSTRACT
We investigated the use of PCR as an alternative to culture of fecal samples for detection of vanA-containing Enterococcus faecium during a recent hospital outbreak. Rectal swabs collected consecutively from 223 patients were analyzed by culture with and without enrichment broth and by vanA-specific PCR of enrichment broth samples. Fifty-five specimens were positive for vanA-containing E. faecium by at least one method. The sensitivities of the vanA-specific PCR assay and agar culture with and without enrichment broth were 94.5, 98, and 89%, respectively. All three methods were 100% specific. Final results were obtained much more rapidly by PCR (within 24 to 30 h of specimen submission) than by the culture methods (4 to 5 days). Thus, PCR is an accurate and rapid alternative to culture for detection of vancomycin-resistant enterococci during hospital outbreaks.
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Protéines bactériennes
/
Vancomycine
/
Réaction de polymérisation en chaîne
/
Infection croisée
/
Épidémies de maladies
/
Infections bactériennes à Gram positif
/
Enterococcus faecium
/
Carbon-oxygen ligases
/
Antibactériens
Type d'étude:
Diagnostic_studies
Limites:
Humans
Langue:
En
Journal:
J Clin Microbiol
Année:
1999
Type de document:
Article
Pays d'affiliation:
Canada