The tissue distribution of the human beta3-adrenoceptor studied using a monoclonal antibody: direct evidence of the beta3-adrenoceptor in human adipose tissue, atrium and skeletal muscle.
Int J Obes Relat Metab Disord
; 23(10): 1057-65, 1999 Oct.
Article
de En
| MEDLINE
| ID: mdl-10557026
ABSTRACT
OBJECTIVE:
To develop a monoclonal antibody that recognises an epitope of the native beta3-adrenoceptor expressed on the extracellular surface of human cells and tissues.DESIGN:
A high affinity monoclonal antibody, Mab72c, was raised against the human beta3-adrenoceptor expressed on a transfected mammalian cell line.RESULTS:
In CHO (Chinese hamster ovary) cells transfected with beta3-adrenoceptor cDNA, antibody labelling was found to be proportional to receptor density measured by the binding of the radiolabelled beta-adrenoceptor antagonist, [125I]-iodocyanopindolol. The use of Mab 72c has demonstrated the expression of the beta3-adrenoceptor in a variety of human tissues, including gall bladder, prostate and colon, where a mRNA signal had been detected previously. This study also provides the first direct demonstration of the expression of beta3-adrenoceptors in human skeletal muscle, atrium and adipose tissue.CONCLUSION:
The development of this antibody represents an important addition to the armentarium of reagents that are available to study the localisation of beta3-adrenoceptors in human tissues.
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Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Tissu adipeux
/
Récepteurs bêta-adrénergiques
/
Muscles squelettiques
/
Atrium du coeur
Limites:
Animals
/
Humans
Langue:
En
Journal:
Int J Obes Relat Metab Disord
Année:
1999
Type de document:
Article
Pays d'affiliation:
Royaume-Uni