Characterization and chromosomal mapping of a human Necdin pseudogene.
Gene
; 245(1): 185-91, 2000 Mar 07.
Article
de En
| MEDLINE
| ID: mdl-10713459
ABSTRACT
The necdin gene is expressed predominantly in postmitotic neurons and encodes a growth suppressor that interacts with the transcription factors E2F1 and p53. Human necdin gene (NDN) is maternally imprinted and located in Prader-Willi syndrome deletion region 15q11.2-q12. We isolated an NDN homologous sequence from a human genomic DNA library. The homologous sequence is overall 83% identical with necdin cDNA sequence, and possesses a short poly(A) stretch at the 3' end and direct repeats at both ends. Expression of the homologous sequence, which lacks a 5' promoter sequence, was undetected in cultured human cell lines. We mapped this sequence to chromosome 12q14-q21.1 by fluorescence in situ hybridization. These characteristics of the NDN-homologous sequence are consistent with those of processed pseudogenes. The information about the necdin pseudogene in the human genome will be useful for genetic studies on NDN-associated neurogenic disorders.
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Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Pseudogènes
/
Molécules d'adhérence cellulaire
Limites:
Humans
Langue:
En
Journal:
Gene
Année:
2000
Type de document:
Article
Pays d'affiliation:
Japon