Dietary nucleotides can up-regulate antigen-specific Th1 immune responses and suppress antigen-specific IgE responses in mice.
Int Arch Allergy Immunol
; 122(1): 33-41, 2000 May.
Article
de En
| MEDLINE
| ID: mdl-10859467
ABSTRACT
BACKGROUND:
It has been reported that dietary nucleotides enhance T helper cell activities. In this study, we have determined the effects of dietary nucleotides on antigen-specific Th1 and Th2 responses and IgE responses.METHODS:
Ovalbumin (OVA)-specific T cell receptor (TCR) transgenic (OVA-TCR Tg) mice, 3 weeks old, were fed a nucleotide-free diet (NT(-) diet) or the NT(-) diet supplemented with dietary nucleotides (NT(+) diet) for 4 weeks. Cytokine production by spleen cells and macrophages obtained from these mice was measured in vitro. BALB/c mice, 3 weeks old, immunized intraperitoneally with OVA adsorbed onto alum, were fed the NT(-) diet or the NT(+) diet for 4 weeks. Serum levels of antigen-specific antibodies in the BALB/c mice were determined by ELISA.RESULTS:
The level of production of antigen-specific interferon-gamma by spleen cells was significantly higher in the OVA-TCR Tg mice fed the NT(+) diet than in the control mice. The levels of secretion of bioactive IL-12 by spleen cells and peritoneal macrophages were also significantly increased in the NT(+) diet group. The serum OVA-specific IgE level was significantly decreased in BALB/c mice fed the NT(+) diet compared with those fed the NT(-) diet.CONCLUSION:
These results show that dietary nucleotides up-regulate the antigen-specific Th1 immune response through the enhancement of IL-12 production and suppress the antigen-specific IgE response.
Recherche sur Google
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Immunoglobuline E
/
Adjuvants immunologiques
/
Lymphocytes auxiliaires Th1
/
Régime alimentaire
/
Spécificité des anticorps
/
Nucléotides
Limites:
Animals
Langue:
En
Journal:
Int Arch Allergy Immunol
Sujet du journal:
ALERGIA E IMUNOLOGIA
Année:
2000
Type de document:
Article
Pays d'affiliation:
Japon