Chronic metabolic acidosis increases NaDC-1 mRNA and protein abundance in rat kidney.
Kidney Int
; 58(1): 206-15, 2000 Jul.
Article
de En
| MEDLINE
| ID: mdl-10886565
ABSTRACT
BACKGROUND:
Chronic metabolic acidosis increases, while alkali feeding inhibits, proximal tubule citrate absorption. The activity of the apical membrane Na+/citrate cotransporter is increased in metabolic acidosis, but is not altered by alkali feeding.METHODS:
Renal cortical mRNA and brush border membrane protein abundances of sodium/dicarboxylate-1 (NaDC-1), the apical membrane Na+/citrate transporter, were measured.RESULTS:
By immunohistochemistry, NaDC-1 was localized to the apical membrane of the proximal tubule. Chronic metabolic acidosis caused an increase in NaDC-1 protein abundance that was maximal in the S2 segment and that increased with time. Metabolic acidosis also increased NaDC-1 mRNA abundance, but this was first seen at three hours and correlated with the severity of the metabolic acidosis. Alkali feeding had no effect on NaDC-1 protein or mRNA abundance.CONCLUSIONS:
Chronic metabolic acidosis increases renal cortical NaDC-1 mRNA abundance and apical membrane NaDC-1 protein abundance, while alkali feeding is without effect on NaDC-1.
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Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Acidose
/
Protéines de transport
/
Transporteurs d'acides dicarboxyliques
/
Transporteurs d'anions organiques sodium-dépendants
/
Symporteurs
/
Tubules contournés proximaux
/
Protéines membranaires
Limites:
Animals
Langue:
En
Journal:
Kidney Int
Année:
2000
Type de document:
Article
Pays d'affiliation:
États-Unis d'Amérique