Real-time quantitation of bcr-abl transcripts in haematological malignancies.
Eur J Haematol
; 65(4): 258-66, 2000 Oct.
Article
de En
| MEDLINE
| ID: mdl-11073166
ABSTRACT
We have applied an automated real-time quantitative PCR assay using a double-labeled fluorogenic probe to detect t(9;22)-positive cells in haematological malignancies. The results are expressed as the ratio of chimeric bcr-abl transcripts on abl transcripts. Highly reproducible results were obtained for t(9;22)-positive K562 RNA. Ten copies of bcr-abl DNA from a recombinant KW-3 plasmid and one positive cell in 10(4) can be detected. Thirty-two patients with chronic myeloid leukaemia (CML), 25 with acute leukaemia, 12 with myelodysplastic syndromes and 7 with other myeloproliferative syndromes were tested. Follow-up data were obtained in bcr-abl positive cases. Results were compared with those of conventional nested RT-PCR and cytogenetics. Real-time quantitative RT-PCR values correlated well with both these methods. However, in some cases the only means of detecting early relapse or blastic transformation was to examine the kinetics of real-time quantitative RT-PCR. Thus, real-time quantitative RT-PCR appears suitable for the diagnosis and follow-up of patients with the t(9;22) translocation.
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Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
ARN messager
/
Protéines de fusion bcr-abl
/
Tumeurs hématologiques
Type d'étude:
Diagnostic_studies
/
Observational_studies
/
Prognostic_studies
Limites:
Female
/
Humans
/
Male
Langue:
En
Journal:
Eur J Haematol
Sujet du journal:
HEMATOLOGIA
Année:
2000
Type de document:
Article
Pays d'affiliation:
France