Determination of the enantiomers of 3-tert.-butylamino-1,2-propanediol by high-performance liquid chromatography using mass spectrometric detection.
J Chromatogr A
; 896(1-2): 201-7, 2000 Oct 27.
Article
de En
| MEDLINE
| ID: mdl-11093655
ABSTRACT
The chiral synthesis of beta-blockers such as (S)-timolol requires a sensitive analytical method for the enantioseparation of its intermediate, 3-tert.-butylamino-1,2-propanediol, in the ng/ml range. The method developed is based on on-line normal-phase LC-MS-MS using a chiral stationary phase and an atmospheric pressure chemical ionization (APCI) interface. The MS detection of 3-tert.-butylamino-1,2-propanediol was first optimized with a pneumatically-assisted electrospray interface (ionspray). The APCI interface was then selected for LC-MS-MS because of the incompatibility of electrospray with n-hexane. The method was validated for both enantiomers in the 25-500 ng/ml concentration range.
Recherche sur Google
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Propylène glycols
/
Chromatographie en phase liquide à haute performance
Type d'étude:
Diagnostic_studies
Langue:
En
Journal:
J Chromatogr A
Année:
2000
Type de document:
Article
Pays d'affiliation:
Belgique