Shear stress-dependent expression of apoptosis-regulating genes in endothelial cells.
Biochem Biophys Res Commun
; 278(3): 740-6, 2000 Nov 30.
Article
de En
| MEDLINE
| ID: mdl-11095978
ABSTRACT
Laminar shear stress exerts potent anti-apoptotic effects. Therefore, we analyzed the influence of laminar shear stress on the expression of apoptosis-regulating genes in human umbilical vein endothelial cells (HUVEC). Application of high levels of laminar shear stress (15 and 30 dyn/cm(2)) decreased the susceptibility of HUVEC to undergo apoptosis, whereas low shear stress (1 dyn/cm(2)) had no effect. These diminished signs of apoptosis were accompanied by a decreased mRNA expression of apoptosis-inducing Fas receptor. Furthermore, mRNA and protein expression of anti-apoptotic, soluble Fas isoform FasExo6Del and anti-apoptotic Bcl-x(L) were induced. Surprisingly, high shear stress also elevated mRNA and protein expression of pro-apoptotic Bak. The shear stress-induced up-regulation of Bcl-x(L) and Bak mRNA can be abrogated by inhibition of the endothelial NO synthase. We propose that altered expression of Bcl-x(L) and the Fas system is involved in the protective effect of laminar shear stress against apoptosis in human endothelial cells.
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Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Endothélium vasculaire
/
Régulation de l'expression des gènes
/
Apoptose
Limites:
Humans
Langue:
En
Journal:
Biochem Biophys Res Commun
Année:
2000
Type de document:
Article
Pays d'affiliation:
Allemagne