Up-regulation of cyclin B1 and cdc2 expression during 9-nitrocamptothecin-induced regression of DU145 prostate tumor.
Anticancer Res
; 20(6B): 4477-82, 2000.
Article
de En
| MEDLINE
| ID: mdl-11205291
ABSTRACT
BACKGROUND:
We investigated changes in the content and subcellular localization of the cell cycle regulators, cyclin B1 and cyclin-dependent kinase cdc2, in human prostate DU145 tumor and cultured cells treated with the anticancer drug 9-nitrocamptothecin (9NC). MATERIALS ANDMETHODS:
Proteins of interest were identified by Western blot methodology using specific antibodies.RESULTS:
The cyclin B1 and cdc2 contents were dramatically elevated in biopsies of DU145 tumor regressing upon 9NC-treatment. In vitro, 9NC-induced apoptosis of DU145 cells was associated with up-regulation of expression and nuclear accumulation of cyclin B1 and cdc2. No changes were observed in cyclins A and E and the cyclin-dependent kinase cdk2 in 9NC-treated DU145 tumor and cultured cells.CONCLUSION:
9NC-induced apoptosis in DU145 cells in vivo and in vitro is associated with up-regulation of expression and nuclear localization of cyclin B1 and cdc2.
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Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Camptothécine
/
Protéine-kinase CDC2
/
Cycline B
/
Kinases CDC2-CDC28
/
Protéines tumorales
/
Antinéoplasiques
Limites:
Humans
Langue:
En
Journal:
Anticancer Res
Année:
2000
Type de document:
Article
Pays d'affiliation:
États-Unis d'Amérique