Morphological aspects of the female pig bladder neck and urethra: quantitative analysis using computer assisted 3-dimensional reconstructions.
J Urol
; 165(4): 1294-9, 2001 Apr.
Article
de En
| MEDLINE
| ID: mdl-11257704
ABSTRACT
PURPOSE:
To investigate whether the pig is a suitable model for studies of lower urinary tract function and dysfunction, we sought to determine the morphology of the female pig bladder neck and urethra. Computer assisted 3-dimensional (D) reconstructions from step serial histological sections were used for visualization of the spatial relationships between neighboring urethral wall components, and the quantification of these components in the bladder neck and along the urethra. MATERIALS ANDMETHODS:
Step serial histological paraffin sections from the bladder neck and urethra of 6 female pigs, stained with Masson's trichrome, were used to generate computer assisted 3-D reconstructions using MacStereology (Ranfurly MicroSystems Ltd., Airdrie, United Kingdom) as the 3-D software package.RESULTS:
The bladder neck and urethral anatomy revealed well defined smooth and striated muscle layers that varied in location, regional distribution and orientation. Circular smooth muscle was maximally developed in the mid urethra, at which point maximal urethral pressure was observed. The longitudinal smooth muscle layer appeared continuous with the detrusor, implicating a possible role in urethral shortening at the onset of voiding. A small circular and longitudinal striated muscle component was present in the distal urethra.CONCLUSIONS:
Anatomical differences exist between the female pig and human bladder neck and urethra, which were successfully highlighted using computer assisted 3-D reconstructions from step serial histological paraffin sections.
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Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Urètre
/
Vessie urinaire
/
Traitement d'image par ordinateur
Limites:
Animals
Langue:
En
Journal:
J Urol
Année:
2001
Type de document:
Article
Pays d'affiliation:
Royaume-Uni