Detection of dengue viruses in field caught male Aedes aegypti and Aedes albopictus (Diptera: Culicidae) in Singapore by type-specific PCR.
J Med Entomol
; 38(4): 475-9, 2001 Jul.
Article
de En
| MEDLINE
| ID: mdl-11476326
ABSTRACT
Field male Aedes aegypti (L.) and Aedes albopictus (Skuse) adults caught from fixed monitoring stations weekly for 1 yr were screened for dengue viruses (DEN-1, DEN-2, DEN-3, and DEN-4). The assay was carried out using a single-step reverse transcription (or transcriptase)-polymerase chain reaction (PCR) (RT-PCR) followed by a semi-nested PCR using an upstream consensus primer and four type-specific primers within the nonstructural protein three gene (NS3) of dengue viruses. The diagnostic fragments for DEN-1, DEN-2, DEN-3, and DEN-4 serotypes were of sizes 169, 362, 265, and 426 bp, respectively. Results showed that in Singapore 1.33% and 2.15% of Aedes aegypti and Aedes albopictus adult male mosquitoes, respectively, were positive for dengue viruses. The serotypes detected in male Ae. aegypti was DEN-1 (44%), followed by DEN-2 (22.2%) and DEN-3 (22.2%), and DEN-4 (11.1%). For Aedes albopictus males, the serotype was DEN-4 (38.9%), followed by DEN-2 (33.3%), DEN-3 (16.7%), and DEN-1 (11.1%).
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Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Aedes
/
Virus de la dengue
Type d'étude:
Diagnostic_studies
Limites:
Animals
/
Humans
/
Male
Pays/Région comme sujet:
Asia
Langue:
En
Journal:
J Med Entomol
Année:
2001
Type de document:
Article
Pays d'affiliation:
Singapour