Superoxide generation from human polymorphonuclear leukocytes by liposome-encapsulated hemoglobin.
Artif Cells Blood Substit Immobil Biotechnol
; 29(4): 275-83, 2001 Jul.
Article
de En
| MEDLINE
| ID: mdl-11495009
ABSTRACT
We investigated the interactions between liposome-encapsulated hemoglobin (Neo Red Cells NRC) and human polymorphonuclear leukocytes as assessed by superoxide generation. NRC triggered superoxide generation from neutrophils in a dose-dependent manner. Empty liposomes also induced superoxide production of neutrophils. Superoxide generation of neutrophils induced by phorbol myristate acetate (PMA) was delayed but intensified both by NRC and empty liposomes. The intensity of superoxide generation induced by NRC was smaller than that by the empty liposomes. As NRC contained superoxide dismutase (SOD) that was copurified with hemoglobin from red blood cells and its activity remained, SOD contained in NRC may partially eliminate superoxide.
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Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Hémoglobines
/
Superoxydes
/
Granulocytes neutrophiles
Limites:
Humans
Langue:
En
Journal:
Artif Cells Blood Substit Immobil Biotechnol
Sujet du journal:
BIOTECNOLOGIA
/
TRANSPLANTE
Année:
2001
Type de document:
Article
Pays d'affiliation:
Japon