Identification and structural analysis of synthetic oligosaccharides of Shigella sonnei using MALDI-TOF MS.
Carbohydr Res
; 334(4): 315-22, 2001 Sep 07.
Article
de En
| MEDLINE
| ID: mdl-11527533
MALDI-TOF mass spectroscopy was used for the molecular weight determination of protected synthetic oligosaccharides related to a cell surface bacterial polysaccharide. By-products containing chlorinated protecting groups caused isotopic patterns characteristic of the natural isotopic distribution of chlorine, were identified on the basis of isotopic distribution. 2,4,6-Trihydroxyacetophenone (THAP) as a matrix was better than 2,5-dihydroxybenzoic acid (DHB) for compounds containing chlorine, since monoisotopic resolution and no fragmentation were observed. In the post source decay (PSD) mode the identification of the oligosaccharide sequence through cleavage of the interglycosidic linkages was also possible, thus providing a sensitive and accurate tool for the structural verification of synthetic oligosaccharide intermediates.
Recherche sur Google
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Oligosaccharides
/
Shigella sonnei
/
Spectrométrie de masse MALDI
Type d'étude:
Diagnostic_studies
/
Prognostic_studies
Langue:
En
Journal:
Carbohydr Res
Année:
2001
Type de document:
Article
Pays d'affiliation:
Hongrie
Pays de publication:
Pays-Bas