Slide-format proteomic biochips based on surface-enhanced nanocluster-resonance.
Fresenius J Anal Chem
; 371(2): 238-45, 2001 Sep.
Article
de En
| MEDLINE
| ID: mdl-11678198
ABSTRACT
The most fundamental properties of metal nanoclusters, namely the high local-field enhancement and nanoscale resonance behavior of the cluster electron plasma when exited by electromagnetic radiation, have been used to set up a variety of sensors transducing biorecognitive interactions into optical signals. This paper focuses on applications of resonant-cluster technology, which enabled us to monitor biorecognitive binding of a variety of proteins on a chip, thus constructing high-throughput interaction-screening devices. Decisive for this type of sensor is the nanometric distance from the local field surrounding a cluster to other parts of the sensor interacting with this field. In particular, the cluster-mirror or cluster-fluorophore distance gives rise to a variety of enhancement phenomena. Depending on the desired application this "resonance"- distance is approximately 5-400 nm. All types of sensor can be set up on photolithographically constructed microchips, but microscopic glass slides can also be employed; this also enables the use of standard devices for dotting and read out. Using slide based chips a standard format of 3,200 microdots (125 microm in diameter) was the basis of either microassays applying direct optical transduction via surfaceenhanced absorption or striking for more sensitivity via surface-enhanced fluorescence.
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Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Résonance plasmonique de surface
/
Protéome
/
Microchimie
Type d'étude:
Diagnostic_studies
Limites:
Animals
/
Humans
Langue:
En
Journal:
Fresenius J Anal Chem
Sujet du journal:
QUIMICA
Année:
2001
Type de document:
Article
Pays d'affiliation:
Pays-Bas