Efficient metaphase II transgenesis with different transgene archetypes.
Nat Biotechnol
; 19(11): 1071-3, 2001 Nov.
Article
de En
| MEDLINE
| ID: mdl-11689854
ABSTRACT
Mammalian genome characterization and biotechnology each require the mobilization of large DNA segments to produce transgenic animals. We recently showed that mouse metaphase II (mII) oocytes could efficiently promote transgenesis (mII transgenesis) when coinjected with sperm and small (<5 kilobases) ubiquitously expressed transgenes (tgs). We have extended this work and now report that mII transgenesis can readily be applied to a range of larger tgs (11.9-170 kilobases), including bacterial and mammalian artificial chromosome (BAC and MAC) constructs. The efficiency of large-construct mII transgenesis was at least as high as that with small constructs; 11-47% of offspring carried the large tgs. More than 95% of these transgenic founders transmitted the tg to offspring. These data demonstrate the ability of mII transgenesis to deliver large tgs efficiently.
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Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Souris transgéniques
Limites:
Animals
Langue:
En
Journal:
Nat Biotechnol
Sujet du journal:
BIOTECNOLOGIA
Année:
2001
Type de document:
Article
Pays d'affiliation:
États-Unis d'Amérique