Virus inactivation in hemoglobin solution by heat treatment.
Artif Cells Blood Substit Immobil Biotechnol
; 29(5): 381-8, 2001 Sep.
Article
de En
| MEDLINE
| ID: mdl-11708661
ABSTRACT
To increase the safety of stroma-free hemoglobin solution (SFH) as an artificial oxygen carrier source, we investigated the effect of heat treatment on virus inactivation in hemoglobin solution. The hemoglobin solution spiked with vesicular stomatitis virus (VSV) was treated at 60 degrees C for 1 hr under either an air or CO atmosphere. VSV was inactivated at >5.8 log10 and >6.0 log10 under the air and CO atmosphere, respectively. Although the methemoglobin rate increased after the heat treatment under the air atmosphere, no methemoglobin formation was observed by the treatment under the CO atmosphere. Isoelectric focusing analysis revealed the denaturation of hemoglobin after the heat treatment under the air, while hemoglobin banding was not altered in the carbonylated condition. Some protein bands other than hemoglobin were weakened or disappeared on SDS-PAGE after the heat treatment under both conditions. In addition, the hemoglobin concentration in the SFH was higher after the heat treatment than before the treatment. These findings indicate that the heat treatment under the CO atmosphere inactivates viruses without hemoglobin denaturation, and hence, this method is a promising approach to prepare a safer SFH as artificial oxygen carriers.
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Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Solutions
/
Hémoglobines
/
Stérilisation
/
Température élevée
Limites:
Humans
Langue:
En
Journal:
Artif Cells Blood Substit Immobil Biotechnol
Sujet du journal:
BIOTECNOLOGIA
/
TRANSPLANTE
Année:
2001
Type de document:
Article
Pays d'affiliation:
Japon