Antisense oligonucleotides specific to mutated K-ras genes inhibit invasiveness of human pancreatic cancer cell lines.
Pancreatology
; 1(4): 314-9, 2001.
Article
de En
| MEDLINE
| ID: mdl-12120210
ABSTRACT
BACKGROUND/AIMS:
Point mutations of the K-ras gene are detected in > 90% of human pancreatic cancers and may play an important role in tumorigenesis. However, correlations between mutant K-ras and the invasive activity of the tumor have remained unclarified.METHODS:
17-merphosphorothioate antisense oligonucleotides targeting K-ras point mutations were transfected into three kinds of human pancreatic cancer cell lines (MIAPaCa-2, PANC-1, and BxPC-3), and the invasive activity was investigated using an in vitro chemoinvasion assay.RESULTS:
Antisense oligonucleotides strongly inhibited the invasive activity of the cell lines with mutant K-ras genes (MIAPaCa-2, PANC-1), but not in that with a wild-type K-ras (BxPC-3).CONCLUSION:
Antisense oligonucleotides specific to mutated K-ras genes inhibited the invasiveness of human pancreatic cancer cell lines. Specific antisense therapy to the point mutation of K-ras might be a new anticancer strategy for pancreatic cancer.
Recherche sur Google
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Tumeurs du pancréas
/
Oligonucléotides antisens
/
Gènes ras
/
Invasion tumorale
Limites:
Humans
Langue:
En
Journal:
Pancreatology
Sujet du journal:
ENDOCRINOLOGIA
/
GASTROENTEROLOGIA
Année:
2001
Type de document:
Article
Pays d'affiliation:
Japon