Comparison of MultiMap and TSP/CONCORDE for constructing radiation hybrid maps.
J Hered
; 94(1): 9-13, 2003.
Article
de En
| MEDLINE
| ID: mdl-12692156
ABSTRACT
Radiation hybrid (RH) map construction allows investigators to locate both type I and type II markers on a given genome map. The process is composed of two steps. The first consists of determining the pattern distribution of a set of markers within the different cell lines of an RH panel. This is mainly done by polymerase chain reaction (PCR) amplification and gel electrophoresis, and results in a series of numbers indicating the presence or the absence of each marker in each cell line. The second step consists of a comparison of these numbers, using various algorithms, to group and then order markers. Because different algorithms may provide (slightly) different orders, we have compared the merits of the MultiMap and TSP/CONCORDE packages using a data set of information currently under analysis for construction of the canine genome RH map.
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Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Logiciel
/
Cartographie par hybrides de radiation
Limites:
Animals
Langue:
En
Journal:
J Hered
Année:
2003
Type de document:
Article
Pays d'affiliation:
France