Monomers and dimers of the RepA protein in plasmid pSC101 replication: domains in RepA.
Proc Natl Acad Sci U S A
; 89(19): 8923-7, 1992 Oct 01.
Article
de En
| MEDLINE
| ID: mdl-1409587
ABSTRACT
The replication of plasmid pSC101 requires the plasmid-encoded protein RepA. This protein has a double role it binds to three directly repeated sequences in the pSC101 origin and promotes replication of the plasmid; it binds to two inversely repeated sequences in its promoter region and regulates its own transcription. A series of RepA protein derivatives carrying deletions of the C-terminal region were assayed for specific binding. We found that the last third of the protein is not needed for binding to the various specific sites. Truncated proteins that still bind can also form heterodimers with a wild-type protein. Analysis of band retardation assays conducted with wild-type and truncated proteins indicates that RepA binds to directly repeated sequences as a monomer and to inversely repeated sequences as a dimer.
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Plasmides
/
Protéines bactériennes
/
Protéines
/
Transactivateurs
/
Helicase
/
Protéines de liaison à l'ADN
/
Réplication de l'ADN
/
Escherichia coli
Langue:
En
Journal:
Proc Natl Acad Sci U S A
Année:
1992
Type de document:
Article
Pays d'affiliation:
Suisse