Immune complexes in blood: a new strategy for the analysis of their antigen portion.
Electrophoresis
; 13(9-10): 718-9, 1992.
Article
de En
| MEDLINE
| ID: mdl-1459098
ABSTRACT
A method is described for the characterization of protein antigens from circulating immune complexes from plasma. Free immunoglobulins G were separated from larger immune complexes by gel filtration with a fast protein liquid chromatographic system. The collected immune complexes were dissociated with 4M urea into antigens and antibodies. With a second column run with 4M urea, antigens smaller than 120 kDa were separated from unloaded antibody fractions. After concentration, they were analyzed by two-dimensional gel electrophoresis.
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Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Complexe antigène-anticorps
/
Antigènes
Type d'étude:
Diagnostic_studies
/
Evaluation_studies
Limites:
Humans
Langue:
En
Journal:
Electrophoresis
Année:
1992
Type de document:
Article
Pays d'affiliation:
Suisse