[Epitope of the catalytic subunit of viscumin, exposed on its interaction with a lipid bilayer]. / Epitop kataliticheskoi sub''edinitsy viskumina, poiavliaiushchiisia pri ee vzaimodeistvii s lipidnym bisloem.
Bioorg Khim
; 29(6): 589-96, 2003.
Article
de Ru
| MEDLINE
| ID: mdl-14743532
ABSTRACT
It was previously shown that the catalytic subunit of the plant toxin viscumin induces aggregation of small unilamellar liposomes and this process is inhibited by the mab_TA7 monoclonal antibody produced to the denatured catalytic subunit of viscumin (Agapov, I.I. et al., FEBS Lett., 1999, vol. 464, pp. 63-66). The interaction of the synthetic F101-T105 and A96-T105 fragments of the viscumin catalytic subunit with the mab_TA7 monoclonal antibody was studied by 1H NMR spectroscopy. The results of this study demonstrated that only the A96-T105 fragment is capable of binding to mab_TA7. A nuclear Overhauser effect observed in the antigen-antibody complex and registered on the resonances of the free peptide and exchanging between the free state and the antibody-bound state was analyzed; the mab_TA7 antigen determinant (H99-T105) was identified; and its conformation and orientation within the complex with the antibody were determined. The English version of the paper Russian Journal of Bioorganic Chemistry, 2003, vol. 29, no. 6; see also http//www.maik.ru.
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Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Protéines végétales
/
Toxines biologiques
/
Préparations à base de plantes
/
Double couche lipidique
/
Épitopes
Langue:
Ru
Journal:
Bioorg Khim
Année:
2003
Type de document:
Article