[The radiolabeling property of oligonucleotide with 99mTc using NHS-MAG3 as a chelator].
Sichuan Da Xue Xue Bao Yi Xue Ban
; 35(2): 169-71, 2004 Mar.
Article
de Zh
| MEDLINE
| ID: mdl-15071906
ABSTRACT
OBJECTIVE:
To explore the radiolabeling property of oligonucleotide with 99mTc using NHS-MAG3 as a bifunctional chelator.METHODS:
Three 15-base single-stranded amine-derivitized oligonucleotides, which were antisense(ASON), sense(SON) and mismatched oligonucleotides(MON) of c-myc oncogene mRNA, were coupled with NHS-MAG3 and labeled with 99mTc. The labeled oligonucleotide was purified by Sephadex G25 column chromatogram, then the stability was evaluated. The labeling efficiency of ON-MAG3 was assessed 15 days, 1 month and 2 months after storage at -20 degrees C. The binding rate of 99mTc-ON with plasma protein was measured by the trichloroacetic acid precipitation method.RESULTS:
The average labeling efficiency of 99mTc-ASON, 99mTc-SON and 99mTc-ON was 68.41%, 66.24% and 69.38% respectively, and the radiochemical purity was 96.98%, 95.34% and 94.62%. 99mTc-ON was stable when placed at room temperature or incubated in human serum at 37 degrees C. The labeling efficiency of ON-MAG3 did not significantly change 2 months after storage at -20 degrees C. The plasma protein binding rate of 99mTc-ON was lower than 13%.CONCLUSION:
99mTc-ON labeled with NHS-MAG3 method showed superior radiochemical characteristics. The labeling efficiency and radiochemical purity were desirable. The label was stable in serum and the binding with plasma protein was low. 99mTc-ON could be a sort of potential radiopharmaceutical for in vivo applications.
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Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Oligonucléotides
/
Succinimides
/
Mertiatide de technétium (99mTc)
/
Radiopharmaceutiques
/
Glycine
/
Marquage isotopique
Limites:
Animals
Langue:
Zh
Journal:
Sichuan Da Xue Xue Bao Yi Xue Ban
Année:
2004
Type de document:
Article
Pays d'affiliation:
Chine