Isolation and characterization of NMSO3-resistant mutants of respiratory syncytial virus.
Antiviral Res
; 61(3): 165-71, 2004 Mar.
Article
de En
| MEDLINE
| ID: mdl-15168797
ABSTRACT
We obtained two mutant strains of respiratory syncytial virus (RSV) which showed resistance against NMSO3 after 15 and 33 passages, respectively, in HEp-2 cells in the presence of 6.8 microM of NMSO3. The EC50 values of NMSO3 for the resistant virus strains were 0.48 and 0.93 microM, that is 4.8-9.3 times higher than that of the parent strain (EC50 = 0.1 microM). The most resistant strain also showed resistance against heparin but was sensitive to dextran sulfate and a polyoxotungstate, PM-523. In order to determine whether the acquisition of resistance to NMSO3 was the result of the accumulation of genetic changes of virus, we sequenced the G- and F-protein genes. In comparison with the standard type of RSV strains, we identified changes of 10 amino acids in the G protein including those at the central conserved segment. However, we did not observe any particular changes in the amino acid sequence of the F-protein of the resistant strains. From these results, we conclude that NMSO3 inhibits the G-protein interaction to the receptor. The mutations in the G-protein may result in the observed phenotypic resistance of RSV towards NMSO3.
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Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Antiviraux
/
Virus respiratoires syncytiaux
/
Acide N-acétyl-neuraminique
/
Résistance virale aux médicaments
/
Lipides
Limites:
Humans
Langue:
En
Journal:
Antiviral Res
Année:
2004
Type de document:
Article
Pays d'affiliation:
Japon