Production of the subdomains of the Plasmodium falciparum apical membrane antigen 1 ectodomain and analysis of the immune response.
Infect Immun
; 72(8): 4464-70, 2004 Aug.
Article
de En
| MEDLINE
| ID: mdl-15271904
ABSTRACT
The apical membrane antigen 1 of Plasmodium falciparum is one of the leading candidate antigens being developed as a vaccine to prevent malaria. This merozoite transmembrane protein has an ectodomain that can be divided into three subdomains (D I, D II, and D III). We have previously expressed a major portion of this ectodomain and have shown that it can induce antibodies that prevent merozoite invasion into red blood cells in an in vitro growth and invasion assay. To analyze the antibody responses directed against the individual subdomains, we constructed six different genes that express each of the domains separately (D I, D II, or D III) or in combination with another domain (D I+II, D II+III, or D I+III). These proteins were purified and used to immunize rabbits to raise construct-specific antibodies. We demonstrated that D I+II induced a significant amount of the growth-inhibitory antibodies active in the growth and invasion assay.
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Plasmodium falciparum
/
Anticorps antiprotozoaires
/
Protéines de protozoaire
/
Protéines membranaires
/
Antigènes de protozoaire
Limites:
Animals
Langue:
En
Journal:
Infect Immun
Année:
2004
Type de document:
Article
Pays d'affiliation:
États-Unis d'Amérique