Crystallization and preliminary X-ray crystallographic analysis of shikimate kinase from Erwinia chrysanthemi.
Acta Crystallogr D Biol Crystallogr
; 53(Pt 5): 612-4, 1997 Sep 01.
Article
de En
| MEDLINE
| ID: mdl-15299895
ABSTRACT
Shikimate kinase from Erwinia chrysanthemi, overexpressed in Escherichia coli has been crystallized by the vapour-diffusion method using sodium chloride as a precipitant. Mass spectrometry was used to confirm the purity of the shikimate kinase and dynamic light scattering was used to assess conditions for the monodispersity of the enzyme. The crystals are tetragonal, space group P4(1)2(1)2 or enantiomorph with cell dimensions a = b = 108.5 and c = 92.8 A (at 100 K). Native crystals diffract to better than 2.6 A on a synchrotron X-ray source. The asymmetric unit is likely to contain two molecules, corresponding to a packing density of 3.6 A(3) Da(-1).
Recherche sur Google
Collection:
01-internacional
Base de données:
MEDLINE
Langue:
En
Journal:
Acta Crystallogr D Biol Crystallogr
Année:
1997
Type de document:
Article
Pays d'affiliation:
Royaume-Uni