Passive stiffness of Drosophila IFM myofibrils: a novel, high accuracy measurement method.
J Muscle Res Cell Motil
; 25(4-5): 359-66, 2004.
Article
de En
| MEDLINE
| ID: mdl-15548865
ABSTRACT
As the smallest muscle-cell substructure that retains the intact contractile apparatus, the single myofibril is considered the optimal specimen for muscle mechanics, although its small size also poses some technical difficulties. Myofibrils from Drosophila indirect flight muscle (IFM) are particularly difficult to study because their high passive stiffness makes them hard to handle, and too resistant to stretch to produce enough elongation for the accurate measurement of sarcomere length change. In this study, we devised a novel method for accurate stiffness measurement of single relaxed myofibrils using microfabricated cantilevers and phase contrast microscopy. A special experimental protocol was developed to minimize errors, and some data analysis strategies were used to identify and exclude spurious data. Remarkably consistent results were obtained from Drosophila IFM myofibrils. This novel, high accuracy method is potentially an effective tool for detecting small passive stiffness change in muscle mutants.
Recherche sur Google
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Sarcomères
/
Drosophila melanogaster
/
Contraction musculaire
/
Myofibrilles
Limites:
Animals
Langue:
En
Journal:
J Muscle Res Cell Motil
Année:
2004
Type de document:
Article
Pays d'affiliation:
États-Unis d'Amérique