[A method for preparation of genomic DNA from Grifola frondosa and construction of a genomic library].
Yi Chuan
; 26(5): 711-3, 2004 Sep.
Article
de Zh
| MEDLINE
| ID: mdl-15640090
ABSTRACT
Grifola frondosa, is a valuable medicinal fungus. High quality total genomic DNA is difficult to prepare due to its high polysaccharide content. A method for the preparation of Grifola frondosa total genomic DNA and construction of Grifola frondosa, genomic library is described. Genomic DNA prepared by this method is digested by Sau3A I restriction enzyme. Constructed genomic library give a titer of 2 x 10(5) transformants/50mg , with a average insert size of 14kb. This has paved way for the cloning of other Grifola frondosa genes and molecular biology studies.
Recherche sur Google
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
ADN fongique
/
Banque génomique
/
Grifola
Langue:
Zh
Journal:
Yi Chuan
Sujet du journal:
GENETICA
Année:
2004
Type de document:
Article
Pays d'affiliation:
Chine