Overexpression of cyclooxygenase-2 in nasopharyngeal carcinoma and association with epidermal growth factor receptor expression.
Arch Otolaryngol Head Neck Surg
; 131(2): 147-52, 2005 Feb.
Article
de En
| MEDLINE
| ID: mdl-15723947
ABSTRACT
OBJECTIVES:
To examine the association between cyclooxygenase-2 (COX-2) expression with epidermal growth factor receptor (EGFR), vascular endothelial growth factor (VEGF), inducible nitric oxide synthase (iNOS), and latent membrane protein 1 (LMP-1) expression and with COX-2 promoter methylation status in primary nasopharyngeal cancer (NPC) tumors and to determine COX-2 promoter methylation status in NPC cell lines.DESIGN:
Retrospective study.SETTING:
Patients with NPC were referred to the Department of Otolaryngology-Head and Neck Surgery for treatment. PATIENTS Formalin-fixed, paraffin-embedded NPC specimens from 42 patients were obtained.INTERVENTIONS:
Immunohistochemical expression of COX-2, EGFR, VEGF, iNOS, and LMP-1 was performed in 42 NPC samples. COX-2 promoter methylation status was studied in 20 separate specimens and in 4 NPC cell lines. MAIN OUTCOMEMEASURES:
(1) COX-2, EGFR, VEGF, iNOS, and LMP-1 expression; and (2) COX-2 promotor methylation status.RESULTS:
COX-2 was overexpressed in 79% of NPC specimens and was associated with EGFR status (P = .03) but not with LMP-1 or iNOS. In primary NPC tissue, methylation of the COX-2 promoter was seen in 4 of 7 COX-2-negative and 1 of 13 COX-2-positive immunohistochemical cases. COX-2 promoter methylation was found in the CNE-1 cell line.CONCLUSIONS:
Nasopharyngeal cancer may be a useful target for selective COX-2 inhibition. The absence of promoter methylation may be a necessary component of COX-2 overexpression, and promoter methylation may be one of the mechanisms that regulate COX-2 expression.
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Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Tumeurs du rhinopharynx
/
Prostaglandin-endoperoxide synthases
/
Récepteurs ErbB
Type d'étude:
Observational_studies
/
Risk_factors_studies
Limites:
Adult
/
Aged
/
Female
/
Humans
/
Male
/
Middle aged
Langue:
En
Journal:
Arch Otolaryngol Head Neck Surg
Sujet du journal:
OTORRINOLARINGOLOGIA
Année:
2005
Type de document:
Article
Pays d'affiliation:
Singapour