CNS activity of Pokeweed anti-viral protein (PAP) in mice infected with lymphocytic choriomeningitis virus (LCMV).
BMC Infect Dis
; 5: 9, 2005 Feb 22.
Article
de En
| MEDLINE
| ID: mdl-15725345
ABSTRACT
BACKGROUND:
Others and we have previously described the potent in vivo and in vitro activity of the broad-spectrum antiviral agent PAP (Pokeweed antiviral protein) against a wide range of viruses. The purpose of the present study was to further elucidate the anti-viral spectrum of PAP by examining its effects on the survival of mice challenged with lymphocytic choriomeningitis virus (LCMV).METHODS:
We examined the therapeutic effect of PAP in CBA mice inoculated with intracerebral injections of the WE54 strain of LCMV at a 1000 PFU dose level that is lethal to 100% of mice within 7-9 days. Mice were treated either with vehicle or PAP administered intraperitoneally 24 hours prior to, 1 hour prior to and 24 hours, 48 hours 72 hours and 96 hours after virus inoculation.RESULTS:
PAP exhibits significant in vivo anti- LCMV activity in mice challenged intracerebrally with an otherwise invariably fatal dose of LCMV. At non-toxic dose levels, PAP significantly prolonged survival in the absence of the majority of disease-associated symptoms. The median survival time of PAP-treated mice was >21 days as opposed to 7 days median survival for the control (p = 0.0069).CONCLUSION:
Our results presented herein provide unprecedented experimental evidence that PAP exhibits antiviral activity in the CNS of LCMV-infected mice.
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Antiviraux
/
Protéines végétales
/
Encéphale
/
Inhibiteurs de la synthèse protéique
/
Chorioméningite lymphocytaire
/
N-Glycosyl hydrolases
Limites:
Animals
Langue:
En
Journal:
BMC Infect Dis
Sujet du journal:
DOENCAS TRANSMISSIVEIS
Année:
2005
Type de document:
Article
Pays d'affiliation:
États-Unis d'Amérique