Enforced Bcl-2 expression overrides serum and feeder cell requirements for mouse embryonic stem cell self-renewal.
Proc Natl Acad Sci U S A
; 102(9): 3312-7, 2005 Mar 01.
Article
de En
| MEDLINE
| ID: mdl-15728354
ABSTRACT
Leukemia inhibitory factor (LIF) is required, but not sufficient, for pluripotent mouse embryonic stem (ES) cell expansion in vitro in the absence of serum or a feeder cell layer, suggesting that additional signals are provided by serum or feeders that are necessary to support self-renewal. Here we show that transgenic ES cell lines expressing Bcl-2, an antiapoptotic protein, continue to self-renew in serum- and feeder-free conditions when supplemented with LIF; even in the absence of bone morphogenic proteins. Bcl-2-expressing clones sustain the characteristics of undifferentiated, pluripotent ES cells during long-term culture, and maintain their potential to differentiate into mature cell types. These results suggest that LIF and Bcl-2 overexpression are sufficient to expand these mouse pluripotent stem cells in vitro.
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Cellules souches
/
Sang
/
Protéines proto-oncogènes c-bcl-2
/
Embryon de mammifère
Limites:
Animals
/
Female
/
Humans
Langue:
En
Journal:
Proc Natl Acad Sci U S A
Année:
2005
Type de document:
Article
Pays d'affiliation:
États-Unis d'Amérique