Cloning and characterization of a cDNA encoding beta-amyrin synthase from petroleum plant Euphorbia tirucalli L.
Phytochemistry
; 66(15): 1759-66, 2005 Aug.
Article
de En
| MEDLINE
| ID: mdl-16005035
ABSTRACT
Euphorbia tirucalli L., known as the petroleum plant, produces a large amount of triterpenes, such as beta-amyrin. Degenerate RT-PCR based on the sequences conserved among known beta-amyrin synthases led to cloning of a putative triterpene synthase cDNA, EtAS, from leaves of E. tirucalli. The deduced amino acid sequence of the EtAS cDNA showed the highest identity of 82% to the Panax ginseng beta-amyrin synthase. Heterologous expression of the EtAS ORF in the methylotrophic yeast, Pichia pastoris, resulted in production of beta-amyrin, revealing that the EtAS cDNA codes for a beta-amyrin synthase. This is the first report of a gene involved in the triterpene synthetic pathway from Euphorbiaceae plants.
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Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Intramolecular transferases
/
Euphorbia
Langue:
En
Journal:
Phytochemistry
Année:
2005
Type de document:
Article
Pays d'affiliation:
Japon