[The effect of Siwu Tang on EPO and G-CSF gene expression in bone marrow of irradiated blood deficiency mice].
Zhongguo Zhong Yao Za Zhi
; 30(15): 1173-6, 2005 Aug.
Article
de Zh
| MEDLINE
| ID: mdl-16201694
ABSTRACT
OBJECTIVE:
To observe the blood enriching function of Siwu Tang and its effect on Epo and G-CSF gene expressions in bone marrow of blood deficiency mice, and thus provide the basis for understanding the molecular mechanism of blood enriching function of Siwu Tang.METHOD:
The animal model of blood deficiency were established in the mice by using 3.5 Gy60Co gamma-ray. Peripheral blood cells were analyzed and CFU-GM, BFU-E, CFU-E and CFU-mix were counted in bone marrow colony cultured. Both Epo and G-CSF gene expressions in bone marrow were measured with RT-PCR.RESULT:
Siwu Tang significantly increased the number of peripheral blood cells and the amount of CFU-GM, BFU-E, CFU-E and CFU-mix in bone marrow and enhanced Epo and G-CSF gene expression in bone marrow in the mice with blood deficiency.CONCLUSION:
The promotion of Epo and G-CSF gene expressions in bone marrow may be one of the mechanisms underlying the blood enriching function of Siwu Tang decoction.
Recherche sur Google
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Moelle osseuse
/
Médicaments issus de plantes chinoises
/
Facteur de stimulation des colonies de granulocytes
/
Érythropoïétine
/
Médecine traditionnelle chinoise
Type d'étude:
Diagnostic_studies
/
Prognostic_studies
Limites:
Animals
Langue:
Zh
Journal:
Zhongguo Zhong Yao Za Zhi
Sujet du journal:
FARMACOLOGIA
/
TERAPIAS COMPLEMENTARES
Année:
2005
Type de document:
Article
Pays d'affiliation:
Chine