Mixed lymphocyte cultures can predict TCR Vbeta repertoires of T cells infiltrating kidney transplants during acute rejection episodes.
Transplantation
; 80(12): 1805-8, 2005 Dec 27.
Article
de En
| MEDLINE
| ID: mdl-16378077
ABSTRACT
Alloreactive T cell populations can show skewing of T-cell antigen receptor (TCR) Vbeta gene usage. The aims of the experiments were to compare in vivo and in vitro T cell alloresponses against donor alloantigens for TCR Vbeta gene usage. T-cell cultures from renal biopsies taken during acute rejection and pretransplant mixed lymphocyte cultures (MLC) were established from five renal transplant patients. TCR Vbeta gene usage, assessed with Vbeta family specific antibodies, showed that up to five different Vbeta families were significantly expanded. In four of five cases, there was close concordance between Vbeta families expanded from the biopsy and in MLC. T-cell clones from one renal biopsy were specific for the mismatched donor alloantigen and showed similar TCR Vbeta gene usage to the original T-cell line. The results show very similar patterns of TCR Vbeta gene usage in alloreactive T cells generated ex vivo or in vitro.
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Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Lymphocytes T
/
Transplantation rénale
/
Récepteur lymphocytaire T antigène, alpha-bêta
/
Test de culture lymphocytaire mixte
/
Rejet du greffon
Type d'étude:
Prognostic_studies
/
Risk_factors_studies
Limites:
Humans
Langue:
En
Journal:
Transplantation
Année:
2005
Type de document:
Article
Pays d'affiliation:
Royaume-Uni