Polar networks control oligomeric assembly in membranes.
J Am Chem Soc
; 128(13): 4170-1, 2006 Apr 05.
Article
de En
| MEDLINE
| ID: mdl-16568959
ABSTRACT
Polar interactions have a profound influence on membrane stability and structure. A membrane-solubilized GCN4 peptide, MS-1, is used to study the impact of polar networks. Amide functionalities from amino acid side chains have been shown to promote peptide oligomerization, but lacked specificity. Herein, the hydrogen bonding interactions of an Asn side chain are coupled with the hydroxyl of Ser or Thr to generate a polar network. Analytical ultracentrifugation and fluorescence resonance energy transfer studies indicate that a trimer assembly is established where each membrane-embedded hydrogen bond contributes 1 kcal mol-1.
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Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Peptides
/
Protéines membranaires
Langue:
En
Journal:
J Am Chem Soc
Année:
2006
Type de document:
Article
Pays d'affiliation:
États-Unis d'Amérique