Reactivity of blood samples spotted onto filter papers in the WST-8 method for screening of G6PD deficiency.
Acta Med Okayama
; 60(2): 127-34, 2006 Apr.
Article
de En
| MEDLINE
| ID: mdl-16680190
ABSTRACT
Deficiency of glucose-6-phosphate dehydrogenase (G6PD) causes acute hemolytic anemia triggered by oxidative drugs such as primaquine. It is therefore essential in malaria-endemic areas for malaria patients to be confirmed for their G6PD activity before taking primaquine. The WST-8 method, a newly established screening method for G6PD deficiency, has been demonstrated to be suitable for field conditions, particularly for on-site malaria surveys. Here we report a laboratory evaluation by this method of the reactivity of blood-spotted filters. A time-course experiment was conducted to evaluate the reactivity of blood samples spotted onto 4 types of filter paper, Whatman 31ET Chr (ET), 3MM Chr (3MM), P81, and Advantec No. 2 (AD2). The rank of the relative reaction intensity was ET > 3MM = AD2 > P81. Blood-spotted filters stored at 4 degrees C gradually decreased G6PD reactivity with the passage of storage time, whereas those stored at room temperature rapidly reduced their reactivity. Unexpectedly, saponin supplementation reduced the reactivity of blood-spotted filters. In conclusion, 1) ET is the most suitable filter for the WST-8 method; 2) blood-spotted filters stored in cold condition can be assayed within 14 days, or those stored at room temperature should be tested within 3 days; and 3) reaction mixtures should not contain saponin.
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Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Sels de tétrazolium
/
Dépistage de masse
/
Glucose 6-phosphate dehydrogenase
/
Déficit en glucose-6-phosphate-déshydrogénase
Type d'étude:
Diagnostic_studies
/
Prognostic_studies
/
Screening_studies
Limites:
Humans
Langue:
En
Journal:
Acta Med Okayama
Année:
2006
Type de document:
Article
Pays d'affiliation:
Japon