Sequencing DNA by dynamic force spectroscopy: limitations and prospects.
Nano Lett
; 6(7): 1483-6, 2006 Jul.
Article
de En
| MEDLINE
| ID: mdl-16834434
ABSTRACT
Using a well established model, we systematically analyze fundamental limitations on the viability of using mechanical unzipping of DNA as a fast and inexpensive sequencing method. Standard unzipping techniques, where double-stranded DNA is unzipped through the application of a force at one end of the molecule, are shown to be inadequate. Emerging techniques that unzip DNA by local force application are more promising, and we establish the necessary experimental requirements that must be met for these techniques to succeed as single molecule sequencing tools.
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Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Analyse de séquence d'ADN
Langue:
En
Journal:
Nano Lett
Année:
2006
Type de document:
Article
Pays d'affiliation:
États-Unis d'Amérique