Analysis of circulating immune complexes isolated from plasma, cerebrospinal fluid and urine.
Electrophoresis
; 12(7-8): 478-86, 1991.
Article
de En
| MEDLINE
| ID: mdl-1717249
ABSTRACT
The protein nature of soluble immune complexes (IC) from fresh plasma, cerebrospinal fluid (CSF), and urine was studied by combining several analytical and biochemical techniques. In plasma and CSF, free immunoglobulins G were separated from larger IC by gel filtration with a fast protein liquid chromatographic system. In urine, IC were separated by precipitation with polyethylene glycol. IC were further purified by protein-A and protein-G affinity chromatography and analyzed by two-dimensional gel electrophoresis. Apart from plasma samples from healthy donors, IC from cases with macrocreatine kinase type 1 and multiple sclerosis were analyzed. For CSF two cases of multiple sclerosis and for urine one case with urinary tract infection are shown. The method can be used for the examination of IC of unknown protein composition in body fluids.
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Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Complexe antigène-anticorps
Limites:
Humans
Langue:
En
Journal:
Electrophoresis
Année:
1991
Type de document:
Article
Pays d'affiliation:
Suisse