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A battery of DNA effect biomarkers to evaluate environmental exposure of Flemish adolescents.
Koppen, G; Verheyen, G; Maes, A; Van Gorp, U; Schoeters, G; Hond, E Den; Staessen, J; Nawrot, T; Roels, H A; Vlietinck, R; Verschaeve, L.
Affiliation
  • Koppen G; Center of Expertise in Environmental Toxicology, Flemish Institute of Technological Research (VITO), Mol, Belgium.
J Appl Toxicol ; 27(3): 238-46, 2007.
Article de En | MEDLINE | ID: mdl-17226746
ABSTRACT
The present paper deals with the evaluation of a battery of genotoxicity biomarkers in healthy Flemish adolescents and their relation with common pollutants occurring in their life environment. DNA damage as reflected by the comet assay appeared to be most sensitive to ozone (partial r(2) = 0.102, p < 0.00001), and to a lesser extent to ortho-cresol (partial r(2) = 0.055; p = 0.001) and 1-hydroxy-pyrene (1-OH-pyrene, partial r(2) = 0.031; p = 0.013). 8-hydroxy-deoxyguanosine (8-OHdG) was only related to ortho-cresol (r(2) = 0.069; p < 0.007). Interestingly, the comet assay results and urinary 8-OHdG concentrations were positively correlated with a Pearson r = 0.21 (p = 0.003, N = 200). Logistic regression models revealed significant relations between chromatid breaks and 1-OH-pyrene (relative risk (RR) 1.58; p = 0.008), and t,t-muconic acid (RR 1.71; p = 0.014). There was no correlation between micronucleus formation or occurrence of chromosomal or chromatid breaks on the one hand and comet or 8-OHdG results on the other hand. Thus, in this study the comet assay on whole blood samples and urine 8-OHdG measurements especially appeared sensitive biomarkers for assessing the genetic effects of environmental pollutants to which adolescents may be exposed.
Sujet(s)
Recherche sur Google
Collection: 01-internacional Base de données: MEDLINE Sujet principal: Altération de l&apos;ADN / Marqueurs biologiques / Exposition environnementale Type d'étude: Etiology_studies / Prognostic_studies Limites: Adolescent / Female / Humans / Male Pays/Région comme sujet: Europa Langue: En Journal: J Appl Toxicol Année: 2007 Type de document: Article Pays d'affiliation: Belgique
Recherche sur Google
Collection: 01-internacional Base de données: MEDLINE Sujet principal: Altération de l&apos;ADN / Marqueurs biologiques / Exposition environnementale Type d'étude: Etiology_studies / Prognostic_studies Limites: Adolescent / Female / Humans / Male Pays/Région comme sujet: Europa Langue: En Journal: J Appl Toxicol Année: 2007 Type de document: Article Pays d'affiliation: Belgique