Use of hematopoietic progenitor cell count on the Sysmex XE-2100 for peripheral blood stem cell harvest monitoring.
Leuk Lymphoma
; 48(1): 89-96, 2007 Jan.
Article
de En
| MEDLINE
| ID: mdl-17327950
ABSTRACT
Successful peripheral blood stem cell (PBSC) collection depends on the timing of apheresis based on CD34+ cell enumeration. Because this analysis is expensive and induces organization difficulties, we evaluated hematopoietic progenitor cell (HPC) quantification on the Sysmex XE-2100 as a surrogate analysis. We tested 157 blood samples for CD34+ cells and HPC counts. We found a good linear correlation between HPC and CD34+ and determined simple rules allowing to use HPC count in daily practice. We set a positive cut-off >30 HPC/mm(3) for allowing PBSC harvest and a negative cut-off at 0 HPC/mm(3) for which collection should be delayed. These two situations accounted for 62% of cases and CD34+ cell count by flow cytometry confirmed HPC result in 95% of cases. Between 0 and 30 HPC/mm3, CD34+ enumeration is required for decision-making. We conclude that HPC count may be a useful surrogate for CD34+ enumeration in PBSC harvest monitoring.
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Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Hémogramme
/
Cellules souches hématopoïétiques
/
Prélèvement d'organes et de tissus
Type d'étude:
Diagnostic_studies
/
Evaluation_studies
Limites:
Humans
Langue:
En
Journal:
Leuk Lymphoma
Sujet du journal:
HEMATOLOGIA
/
NEOPLASIAS
Année:
2007
Type de document:
Article
Pays d'affiliation:
France