High resolution small animal single photon emission computed tomography: uptake of [99mTc]bombesin and [123I]ioflupane by rat brain.
Q J Nucl Med Mol Imaging
; 51(2): 204-10, 2007 Jun.
Article
de En
| MEDLINE
| ID: mdl-17420719
ABSTRACT
AIM:
The aims of this study were 1) to perform brain single photon emission computed tomography (SPECT) in anesthetized rats with high resolution cameras (HRC) equipped with parallel hole collimation resolution of about 1 mm (HRC1) and 2 mm (HRC2); 2) to assess when and with which radio-tracer HRC1 SPECT shows advantages over HRC2.METHODS:
We used two multicrystal HRCs with parallel square hole collimators, whose pure tungsten septa closely fit the crystals, in turn matched with a 4 inch2 position sensitive photomultiplier. HRC1 showed 1.1 mm and HCR2 2.1 mm resolution at collimator contact. HRCs performed 180 degrees semi-circular orbits around the head of rats image reconstruction occurred with ordered subsets expectation maximization algorithms. Resolution of SPECT was measured with a Derenzo Phantom, resulting 1.4 mm for HRC1 and 2.3 mm for HRC2. Three rats were studied with [(99m)Tc]HMPAO, 3 rats with [(99m)Tc]bombesin (BN) and 48 h later with [(123)I]ioflupane (DaTSCAN). SPECT studies were reviewed by two experienced operators.RESULTS:
Technetium-99m-HMPAO SPECT showed similar images with HRC1 and HRC2. The uptake of BN by amygdale, hippocampus and olfactory tract was detected by both cameras. DaTSCAN SPECT with HRC1 showed detailed image of the tail of the caudatus this image was not obtained with HRC2. DaTSCAN and BN SPECT showed amygdale with both HRCs. However, only the central nucleus of amygdale takes up DaTSCAN, whereas central, lateral and basolateral amygdaloid nuclei express BN receptors. Only HRC1 SPECT showed amygdale larger with BN than with DaTSCAN.CONCLUSION:
Spatial resolution of 1.4 mm is appropriate to detect selected subcerebral structures.
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Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Encéphale
/
Bombésine
/
Composés organiques du technétium
/
Tomographie par émission monophotonique
/
Nortropanes
Type d'étude:
Evaluation_studies
Limites:
Animals
Langue:
En
Journal:
Q J Nucl Med Mol Imaging
Sujet du journal:
MEDICINA NUCLEAR
Année:
2007
Type de document:
Article
Pays d'affiliation:
Italie