[Purification of human VLDL apolipoproteins by middle pressure liquid chromatography].
Sichuan Da Xue Xue Bao Yi Xue Ban
; 38(2): 328-30, 333, 2007 Mar.
Article
de Zh
| MEDLINE
| ID: mdl-17441362
ABSTRACT
OBJECTIVE:
To purify human VLDL apolipoproteins by middle-pressure liquid chromatography.METHODS:
Human VLDLs were isolated by one step density ultracentrifugation. Delipided human VLDL was separated by Sephacryl S-200 molecular sieve chromatography. ApoE was purified by heparin Sepharose CL-6B affinity chromatography. ApoC I ,C II and C III were purified from apoC. fraction by DEAE-Sephacel ion exchange chromatographyRESULTS:
Purified apoE, apoC I, apoC II and apoC III were obtained. SDS-PAGE and immunodiffusion tests indicated the isolated proteins were pure.CONCLUSION:
We have established a purification procedure for human VLDL apolipoproteins with highly efficiency and simplicity by MPLC.
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Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Apolipoprotéines
/
Pression
/
Chromatographie en phase liquide
/
Lipoprotéines VLDL
Limites:
Humans
Langue:
Zh
Journal:
Sichuan Da Xue Xue Bao Yi Xue Ban
Année:
2007
Type de document:
Article
Pays d'affiliation:
Chine