Regulation of cystathionine gamma-lyase in Saccharomyces cerevisiae.
Yeast
; 7(8): 843-8, 1991 Nov.
Article
de En
| MEDLINE
| ID: mdl-1789005
ABSTRACT
Regulation of the two enzymes in reverse trans-sulfuration was investigated in Saccharomyces cerevisiae. In wild-type strains, cystathionine gamma-lyase, but not cystathionine beta-synthase, was depressed nearly 15-fold if cells were starved for both inorganic and organic sulfur compounds. In a met17 strain which is defective of O-acetylserine and O-acetylhomoserine sulfhydrylase, the same enzyme was derepressed if organic sulfur compounds were limited; the repressive effect was in the order of glutathione greater than methionine greater than cysteine. The repressive effect of methionine was not observed, however, in a cys2 cys4 strain which is deficient of serine O-acetyltransferase and cystathionine beta-synthase, indicating that methionine itself is not the effector. The weak repressive effect of cysteine was attributed to inefficient uptake of this amino acid. Our observations indicate that cystathionine gamma-lyase is the target of regulation in reverse trans-sulfuration and that cysteine is very likely to be the effector of this regulation.
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Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Saccharomyces cerevisiae
/
Cystathionine gamma-lyase
Langue:
En
Journal:
Yeast
Sujet du journal:
MICROBIOLOGIA
Année:
1991
Type de document:
Article
Pays d'affiliation:
Japon