Environmental monitoring of Mycobacterium bovis in badger feces and badger sett soil by real-time PCR, as confirmed by immunofluorescence, immunocapture, and cultivation.
Appl Environ Microbiol
; 73(22): 7471-3, 2007 Nov.
Article
de En
| MEDLINE
| ID: mdl-17905874
ABSTRACT
Real-time PCR was used to detect and quantify Mycobacterium bovis cells in naturally infected soil and badger feces. Immunomagnetic capture, immunofluorescence, and selective culture confirmed species identification and cell viability. These techniques will prove useful for monitoring M. bovis in the environment and for elucidating transmission routes between wildlife and cattle.
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Microbiologie du sol
/
Réaction de polymérisation en chaîne
/
Mustelidae
/
Fèces
/
Mycobacterium bovis
Limites:
Animals
Langue:
En
Journal:
Appl Environ Microbiol
Année:
2007
Type de document:
Article
Pays d'affiliation:
Royaume-Uni