Evidence of thermostable amylolytic activity from Rhizopus microsporus var. rhizopodiformis using wheat bran and corncob as alternative carbon source.
Bioprocess Biosyst Eng
; 31(4): 329-34, 2008 Jun.
Article
de En
| MEDLINE
| ID: mdl-17940804
ABSTRACT
Rhizopus microsporus var. rhizopodiformis produced high levels of alpha-amylase and glucoamylase under solid state fermentation, with several agricultural residues, such as wheat bran, cassava flour, sugar cane bagasse, rice straw, corncob and crushed corncob as carbon sources. These materials were humidified with distilled water, tap water, or saline solutions--Segato Rizzatti (SR), Khanna or Vogel. The best substrate for amylase production was wheat bran with SR saline solution (12 v/v). Amylolytic activity was still improved (14.3%) with a mixture of wheat bran, corncob, starch and SR saline solution (110.34.6 w/w/w/v). The optimized culture conditions were initial pH 5, at 45 degrees C during 6 days and relative humidity around 76%. The crude extract exhibited temperature and pH optima around 65 degrees C and 4-5, respectively. Amylase activity was fully stable for 1 h at temperatures up to 75 degrees C, and at pH values between 2.5 and 7.5.
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Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Triticum
/
Fibre alimentaire
/
Techniques de culture cellulaire
/
Bioréacteurs
/
Zea mays
/
Amylases
Langue:
En
Journal:
Bioprocess Biosyst Eng
Sujet du journal:
BIOTECNOLOGIA
/
ENGENHARIA BIOMEDICA
Année:
2008
Type de document:
Article
Pays d'affiliation:
Brésil