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Interaction of suplatast tosilate (IPD) with chloride channels in human blood eosinophils: a potential mechanism underlying its anti-allergic and anti-asthmatic effects.
Agrawal, D K; Cheng, G; Kim, M-J; Kiniwa, M.
Affiliation
  • Agrawal DK; Creighton University School of Medicine, Omaha, NE 68178, USA. dkagr@creighton.edu
Clin Exp Allergy ; 38(2): 305-12, 2008 Feb.
Article de En | MEDLINE | ID: mdl-18028459
ABSTRACT

INTRODUCTION:

Alterations in chloride ion channels have been implicated in the induction of changes in cell shape and volume. Because blood and tissue eosinophilia are hallmarks of bronchial asthma, in this study we examined the role of chloride channels in the underlying effects of suplatast tosilate (IPD), an anti-allergic drug, in human blood eosinophils.

METHODS:

Eosinophils were isolated and purified from the blood of allergic asthmatic donors. Chloride ion currents were recorded using the whole-cell patch-clamp technique in freshly isolated eosinophils. The current-voltage relationship of whole-cell currents in human blood eosinophils was calculated and recorded. The effect of chloride channel blockers was examined on superoxide release, eosinophil chemotaxis as measured by the Boyden chamber, and eosinophil adhesion to endothelial cells. Radioligand binding studies with [3H]IPD and competition curves with chloride channel blockers were performed.

RESULTS:

IPD increased both inward and outward chloride currents in human blood eosinophils. IPD in 1 ng/mL did not have significant effect on chloride current. However, at 5 ng/mL IPD activated both outward and inward currents in human blood eosinophils. Chloride channel blockers inhibited IPD-induced respiratory burst in eosinophils, eosinophil chemotaxis, and eosinophil adhesion to endothelial cells. All these effects of IPD on chloride current and the resultant functional responses in human blood eosinophils were not due to its basic salt, p-toluenesulphonic acid monohydrate. Human blood eosinophils contained specific binding sites for [3H]IPD with K(D) and B(max) values of 187.7+/-105.8 nm and 58.7+/-18.7 fmol/10(6) cells, respectively. Both NPPB and DIDS competed, in a dose-dependent manner, for the specific binding of [3H]IPD in human blood eosinophils.

CONCLUSION:

These data suggest that the anti-allergic and anti-asthmatic effects of IPD could be due to its interaction with chloride channels in human blood eosinophils.
Sujet(s)
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Collection: 01-internacional Base de données: MEDLINE Sujet principal: Composés de sulfonium / Canaux chlorure / Arènesulfonates / Antiasthmatiques / Antiallergiques / Granulocytes éosinophiles Limites: Adult / Aged / Female / Humans / Male / Middle aged Langue: En Journal: Clin Exp Allergy Sujet du journal: ALERGIA E IMUNOLOGIA Année: 2008 Type de document: Article Pays d'affiliation: États-Unis d'Amérique
Recherche sur Google
Collection: 01-internacional Base de données: MEDLINE Sujet principal: Composés de sulfonium / Canaux chlorure / Arènesulfonates / Antiasthmatiques / Antiallergiques / Granulocytes éosinophiles Limites: Adult / Aged / Female / Humans / Male / Middle aged Langue: En Journal: Clin Exp Allergy Sujet du journal: ALERGIA E IMUNOLOGIA Année: 2008 Type de document: Article Pays d'affiliation: États-Unis d'Amérique