Species-specific deletion of the viral attachment glycoprotein of avian metapneumovirus.
Virus Res
; 132(1-2): 114-21, 2008 Mar.
Article
de En
| MEDLINE
| ID: mdl-18160118
ABSTRACT
The avian metapneumovirus (AMPV) genome encodes the fusion (F), small hydrophobic (SH), and attachment glycoprotein (G) as envelope glycoproteins. The F and G proteins mainly function to allow viral entry into host cells during the early steps of the virus life cycle. The highly variable AMPV G protein is a major determinant for distinguishing virus subtypes. Sequence analysis was used to determine if any differences between avian or mammalian cell propagated subtype C AMPV could be detected for the 1.8kb G gene. As a result, the complete 1.8kb G gene was found to be present when AMPV was propagated in our immortal turkey turbinate (TT-1) cell line regardless of passage number. Surprisingly, AMPV propagated for 15 or more passages in mammalian Vero cells revealed an essentially deleted G gene in the viral genome, resulting in no G gene mRNA expression. Although the Vero cell propagated AMPV genome contained a small 122 nucleotide fragment of the G gene, no other mRNA variants were detected from either mammalian or avian propagated AMPV. The G gene truncation might be caused by cellular molecular mechanisms that are species-specific. The lack of viral gene deletions suggests that avian cell propagated AMPV will provide a better alternative host for live recombinant vaccine development based on a reverse genetics system.
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Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Protéines de l'enveloppe virale
/
Délétion de séquence
/
Metapneumovirus
/
Attachement viral
Limites:
Animals
Langue:
En
Journal:
Virus Res
Sujet du journal:
VIROLOGIA
Année:
2008
Type de document:
Article
Pays d'affiliation:
États-Unis d'Amérique