Your browser doesn't support javascript.
loading
Species-specific deletion of the viral attachment glycoprotein of avian metapneumovirus.
Kong, Byung-Whi; Foster, Linda K; Foster, Douglas N.
Affiliation
  • Kong BW; Department of Poultry Science, University of Arkansas, Fayetteville, AR 72701, USA.
Virus Res ; 132(1-2): 114-21, 2008 Mar.
Article de En | MEDLINE | ID: mdl-18160118
ABSTRACT
The avian metapneumovirus (AMPV) genome encodes the fusion (F), small hydrophobic (SH), and attachment glycoprotein (G) as envelope glycoproteins. The F and G proteins mainly function to allow viral entry into host cells during the early steps of the virus life cycle. The highly variable AMPV G protein is a major determinant for distinguishing virus subtypes. Sequence analysis was used to determine if any differences between avian or mammalian cell propagated subtype C AMPV could be detected for the 1.8kb G gene. As a result, the complete 1.8kb G gene was found to be present when AMPV was propagated in our immortal turkey turbinate (TT-1) cell line regardless of passage number. Surprisingly, AMPV propagated for 15 or more passages in mammalian Vero cells revealed an essentially deleted G gene in the viral genome, resulting in no G gene mRNA expression. Although the Vero cell propagated AMPV genome contained a small 122 nucleotide fragment of the G gene, no other mRNA variants were detected from either mammalian or avian propagated AMPV. The G gene truncation might be caused by cellular molecular mechanisms that are species-specific. The lack of viral gene deletions suggests that avian cell propagated AMPV will provide a better alternative host for live recombinant vaccine development based on a reverse genetics system.
Sujet(s)
Recherche sur Google
Collection: 01-internacional Base de données: MEDLINE Sujet principal: Protéines de l'enveloppe virale / Délétion de séquence / Metapneumovirus / Attachement viral Limites: Animals Langue: En Journal: Virus Res Sujet du journal: VIROLOGIA Année: 2008 Type de document: Article Pays d'affiliation: États-Unis d'Amérique
Recherche sur Google
Collection: 01-internacional Base de données: MEDLINE Sujet principal: Protéines de l'enveloppe virale / Délétion de séquence / Metapneumovirus / Attachement viral Limites: Animals Langue: En Journal: Virus Res Sujet du journal: VIROLOGIA Année: 2008 Type de document: Article Pays d'affiliation: États-Unis d'Amérique
...